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The use of a hydrophobic stationary phase is essentially the reverse of normal phase chromatography since the polarity of the mobile and stationary phases have been inverted. Reversed-phase chromatography is a technique using alkyl chains covalently bonded to the stationary phase particles in order to create a hydrophobic stationary phase which has a. Normal Phase Hplc Column And Reverse Phase Hplc Column Column Reverse Functional Group However the distinction depends on the relative polarity of the two phases. . Separate identify and quantify components in a mixture with our supply of HPLC solvents. Acetonitrile and water were continuously stripped by a stream of helium and mixed. Normal-phase chromatography combines a non-polar stationary phase with a polar mobile phase. Two high-performance liquid chromatography HPLC procedures a rapid normal-phase isocratic method for the analysis primarily of retinol and retinoic acid

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